ABSTRACT
This book charts the publishing industry and bestselling fiction from 1900, featuring a comprehensive list of all bestselling fiction titles in the UK. This third edition includes a new introduction which features additional information on current trends in reading including the rise of Black, Asian and LGBTQIA+ publishing;the continuing importance of certain genres and up to date trends in publishing, bookselling, library borrowing and literacy. There are sections on writing for children, on the importance of audiobooks and book clubs, self- published bestsellers as well as many new entries to the present day including bestselling authors such as David Walliams, Peter James, George R R Martin and far less well known authors whose books s sell in their thousands. This is the essential guide to best-selling books, authors, genres, publishing and bookselling since 1900, providing a unique insight into more than a century of entertainment, and opening a window into the reading habits and social life of the British from the death of Queen Victoria to the Coronavirus Pandemic. © Clive Bloom 2002, 2008, 2021. All rights reserved.
ABSTRACT
El presente artículo indaga por la recurrencia de las pandemias en la sociedad contemporánea y sus impactos, a propósito de la emergencia sanitaria experimentada en el mundo por cuenta de la pandemia del Covid-19 en Colombia y el mundo. Para tal fin, se realiza un análisis del impacto de las pandemias con cierta trazabilidad histórica, seguido de una exploración teórica de la coyuntura actual, observada a través de la noción del eterno retorno planteada por el filósofo alemán Friedrich Nietzsche y las perspectivas de algunos filósofos contemporáneos acerca de este fenómeno que ha impactado la vida de todos los individuos y que, sin duda, sugiere enormes cambios y desafíos para el desarrollo de la vida futuraAlternate : This article investigates the recurrence of pandemics in contemporary society and their impacts, regarding the health emergency experienced in the world due to the Covid-19 pandemic in Colombia and the world. For this purpose, an analysis of the impact of pandemics with certain historical traceability is carried out, followed by a theoretical exploration of the current situation, observed through the notion of eternal return raised by the German philosopher Friedrich Nietzsche and the perspectives of some philosophers contemporaries about this phenomenon that has impacted the lives of all individuals and that, without a doubt, suggests enormous changes and challenges for the development of future life
ABSTRACT
Aim: The confirmation of lipolytic activity and role of Rv1900c in the Mycobacterium physiology Methods:rv1900c/N-terminus domain (rv1900NT) were cloned in pET28a/Escherichia coli, purified by affinity chromatography and characterized. Results: A zone of clearance on tributyrin-agar and activity with pNP-decanoate confirmed the lipolytic activity of Rv1900c. The Rv1900NT demonstrated higher enzyme specific activity, Vmax and kcat, but Rv1900c was more thermostable. The lipolytic activity of Rv1900c decreased in presence of ATP. Mycobacterium smegmatis expressed rv1900c/rv1900NT-altered colony morphology, growth, cell surface properties and survival under stress conditions. The effect was more prominent with Rv1900NT as compared with Rv1900c. Conclusion: The study confirmed the lipolytic activity of Rv1900c and suggested its regulation by the adenylate cyclase domain and role in the intracellular survival of bacteria.
Lay abstract Tuberculosis (TB) remains the top contagious/infectious killer in the world. It is caused by the bacteria Mycobacterium tuberculosis. The bacteria resides/replicates in the immune cell that normally has to eradicate infectious microorganisms. Though the treatment of TB is available, the emergence of drug-resistant bacteria is of major concern. The treatment of drug-resistant TB has been reported to be more difficult due to lengthy and complex treatment regimens. Therefore, there is an urgent need for new and better drugs to treat TB/drug-resistant TB. For this purpose understanding the role of each protein in the physiology of mycobacteria is required. Lipids play a critical role in the intracellular survival of this pathogen in the host. Our study demonstrated that LipJ supported the intracellular survival of bacteria. Therefore, it could be a potential drug target.